Insulin-like Growth Factor 1 Long R3 (IGF-1 LR3) is a synthetic IGF-1 analog studied for its speculated stability and bioactivity compared to native IGF-1.

This peptide, modified with a substitution of arginine for glutamic acid at position three and an extended N-terminal peptide sequence, has garnered attention for its theoretical implications in various fields of biological research.

This article delves into the structural characteristics, potential mechanisms of action, and speculative implications of IGF-1 LR3 in cellular growth, differentiation, and metabolic regulation.

Introduction

IGF-1 LR3, a possible derivative of the naturally occurring IGF-1, is believed to exhibit a significantly longer half-life due to modifications that support its resistance to enzymatic degradation.

This extended stability positions IGF-1 LR3 as a subject of interest in research focusing on cellular and molecular biology. The peptide’s potential to interact with IGF-1 receptors and its potential influence on various signaling pathways are aspects that researchers are exploring to uncover its broader implications.

Structural Characteristics

IGF-1 LR3 is an analog of IGF-1 with two primary modifications: a substitution of arginine for glutamic acid at position three and a 13-amino acid extension at the N-terminus. These alterations may result in a substantial increase in its biological activity and half-life. The modified structure potentially allows for a stronger and more prolonged interaction with the IGF-1 receptor, which might be pivotal in amplifying its impacts within the organism.

Mechanisms of Action

Studies suggest that IGF-1 LR3 might influence the binding mechanisms to the IGF-1 receptor, a transmembrane receptor with intrinsic tyrosine kinase activity. Upon binding, the receptor undergoes autophosphorylation, initiating a cascade of downstream signaling events. These pathways include the phosphatidylinositol 3-kinase (PI3K)-Akt pathway and the mitogen-activated protein kinase (MAPK) pathway. Activation of these pathways is theorized to play a role in cellular proliferation, differentiation, and survival.

IGF-1 LR3 Peptide: Cellular Processes

IGF 1 LR3 is hypothesized to support cellular growth and differentiation more effectively than native IGF-1. By activating the IGF-1 receptor, the peptide might stimulate anabolic processes in various cell types. For instance, IGF-1 LR3 might theoretically promote differentiation into mature muscle fibers in myoblasts, suggesting potential implications in muscle biology research. Additionally, it has been hypothesized to influence osteoblast activity, thereby impacting bone formation and maintenance.

IGF-1 LR3 Peptide: Metabolism

Another area of interest is the peptide’s possible role in metabolic regulation. Research indicates that IGF-1 LR3 might influence glucose uptake and lipid metabolism by interacting with the IGF-1 receptor. Investigations purport that this peptide may support glucose transport into cells by upregulating the expression of glucose transporter proteins, which might have implications for understanding metabolic disorders. Similarly, it might modulate lipid metabolism by affecting lipid synthesis and degradation enzymes.

IGF-1 LR3 Peptide: Tissue Engineering

Findings imply that tissue engineering is a field where IGF-1 LR3’s characteristics might be particularly relevant. Its potential to promote cell proliferation and differentiation suggests it might support the growth of engineered tissues. For example, in cartilage tissue engineering, IGF-1 LR3 seems to stimulate the proliferation of chondrocytes and their synthesis of extracellular matrix components, which are considered essential for creating functional cartilage.

IGF-1 LR3 Peptide: Neurobiology and Cognitive Implications

Investigations purport that IGF-1 LR3 might also play a role in neurobiology. It has been hypothesized that the peptide might support neuronal growth and survival, which might be crucial for research into neurodegenerative conditions. By potentially enhancing neurogenesis and synaptic plasticity, IGF-1 LR3 might be explored for its theoretical implications in cognitive function and brain function.

IGF-1 LR3 Peptide: Skeletal Muscle Research

IGF-1 LR3 is theorized to be a potent agent for studying muscle hypertrophy and regeneration in skeletal muscle research. Studies postulate that the peptide may influence muscle satellite cells, which are deemed essential for muscle repair and growth. By promoting the proliferation and differentiation of these cells, IGF-1 LR3 appears useful in understanding mechanisms underlying muscle adaptation and recovery.

IGF-1 LR3 Peptide: Cancer Cells

The peptide’s interaction with growth pathways has led to speculation about its potential implications in cancer research. While the proliferative impacts of IGF-1 LR3 might be relevant in regenerative research, they also raise questions about its role in tumorigenesis. Understanding how IGF-1 LR3 might influence cell cycle regulation and apoptosis might provide insights into cancer biology and potential research targets.

Challenges and Future Directions

Despite its potential implications, exploring IGF-1 LR3 in research comes with challenges. Its  perceived potent biological activity necessitates careful consideration of experimental design to avoid unintended outcomes. Future investigations should focus on comprehending the precise molecular mechanisms of IGF-1 LR3 and its long-term action.

Conclusion

IGF-1 LR3 is a synthetic peptide with a unique structure that may support its stability and bioactivity compared to native IGF-1. Its potential to influence cellular growth, differentiation, and metabolic regulation makes it a subject of interest in various fields of biological research. While the peptide’s properties suggest numerous speculative implications, further investigations are essential to fully understand its mechanisms of action and potential impacts. As research progresses, IGF-1 LR3 might offer valuable insights into cellular and molecular processes, paving the way for new scientific discoveries. Buy peptides from Biotech Peptides if you are a licensed professional interested in high-quality compounds.

References

[i] Jonker SS, Giraud GD, Chang EI, Elman MR, Louey S. Coronary vascular growth matches IGF-1-stimulated cardiac growth in fetal sheep. FASEB J. 2020 Aug;34(8):10041-10055. doi: 10.1096/fj.202000215R. Epub 2020 Jun 23. PMID: 32573852; PMCID: PMC7688557.

[ii] Stremming J, Heard S, White A, Chang EI, Shaw SC, Wesolowski SR, Jonker SS, Rozance PJ, Brown LD. IGF-1 infusion to fetal sheep increases organ growth but not by stimulating nutrient transfer to the fetus. Am J Physiol Endocrinol Metab. 2021 Mar 1;320(3):E527-E538. doi: 10.1152/ajpendo.00453.2020. Epub 2021 Jan 11. PMID: 33427051; PMCID: PMC7988781.

[iii] Stremming J, White A, Donthi A, Batt DG, Hetrick B, Chang EI, Wesolowski SR, Seefeldt MB, McCurdy CE, Rozance PJ, Brown LD. Sheep recombinant IGF-1 promotes organ-specific growth in fetal sheep. Front Physiol. 2022 Aug 25;13:954948. doi: 10.3389/fphys.2022.954948. PMID: 36091374; PMCID: PMC9452821.

[iv] White A, Stremming J, Brown LD, Rozance PJ. Attenuated glucose-stimulated insulin secretion during an acute IGF-1 LR3 infusion into fetal sheep does not persist in isolated islets. J Dev Orig function Dis. 2023 Jun;14(3):353-361. doi: 10.1017/S2040174423000090. Epub 2023 Apr 28. PMID: 37114757; PMCID: PMC10205682.

[v] Lu Z, Liu N, Huang H, Wang Y, Tu T, Qin X, Wang X, Zhang J, Su X, Tian J, Bai Y, Luo H, Yao B, Zhang H. Recombinant expression of IGF-1 and LR3 IGF-1 fused with xylanase in Pichia pastoris. ApplMicrobiolBiotechnol. 2023 Jul;107(14):4543-4551. doi: 10.1007/s00253-023-12606-0. Epub 2023 Jun 1. PMID: 37261455.